Strategies for the production of high-content fructo-oligosaccharides through the removal of small saccharides by co-culture or successive fermentation with yeast.
نویسندگان
چکیده
Fructo-oligosaccharides (FOS) obtained by fermentation of sucrose may be purified at large-scale by continuous chromatography (Simulated Moving Bed: SMB). In order to improve the efficiency of the subsequent SMB purification, the optimization of the fermentative broth composition in salts and sugars was investigated. Fermentations conducted at reduced amount of salts, using Aureobasidium pullulans whole cells, yielded 0.63 ± 0.03 g of FOS per gram of initial sucrose. Additionally, a microbial treatment was proposed to reduce the amount of small saccharides in the mixture. Two approaches were evaluated, namely a co-culture of A. pullulans with Saccharomyces cerevisiae; and a two-step fermentation in which FOS were first synthesized by A. pullulans and then the small saccharides were metabolized by S. cerevisiae. Assays were performed in 100mL shaken flasks and further scaled-up to a 3 L working volume bioreactor. Fermentations in two-step were found to be more efficient than the co-culture ones. FOS were obtained with a purity of 81.6 ± 0.8% (w/w), on a dry weight basis, after the second-step fermentation with S. cerevisiae. The sucrose amount was reduced from 13.5 to 5.4% in total sugars, which suggests that FOS from this culture broth will be more efficiently separated by SMB.
منابع مشابه
Utilization of date syrup as a substrate for carotenoid production by Rhodotorula glutinis
The potential use of date syrup, for the production of carotenoids by Rhodotorula glutinisin batch fermentation process, was investigated during 7 days. The results revealed that carbon (glucose or date syrup) and nitrogen sources [yeast extract, (NH4)2SO4 and NH4NO3] had a significant influence on biomass and carotenoid production. Maximum yield of total carotenoid production (7.94 mg/L) with ...
متن کاملSupplemental Chromium Yeast and / orMannan Oligosaccharides in Growing Quail Diets
To evaluatethe effects of supplementing diets of growing Japanese quails (n=450; 7 days old) with chromium (Cr) yeast and / or mannan oligosaccharides (MOS) on productive performance, carcass traits and the immune system are reported. Diets were a basal diet (control) or the basal diet supplemented with 600 mg Cr-yeast/kg (Cr 1), 1200 mg Cr-yeast/kg (Cr 2), 1.0 g MOS/kg, Cr 1 + MOS or Cr 2 + MO...
متن کاملUse of Yeast Culture in the TMR of Dairy Holstein Cows
The aim of this study was to determine the effect of yeast culture (Saccharomyces cerevisiae SC 47) addition in the diet of dairy cows on their rumen fermentation and milk production. Animals received a diet TMR based on good maize silage with a higher dry matter content (14 kg), 14 kg of lucerne-grass haylage, 5 kg of crushed ears of maize, 5 kg of beet pulp silage, 3 kg of crimped wheat, 2 kg...
متن کاملStatistical Optimization of Arachidonic Acid Production by Mortierella alpina CBS 754.68 in Submerged Fermentation
Arachidonic acid is an essential fatty acid in human nutrition. In the present study, production of arachidonicacid by Mortierella alpina CBS 754.68 was evaluated in submerged fermentation. The fermentation variableswere selected in accordance with the Plackett-Burman (PB) design and further optimized via response surface methodology (RSM). Five significant variables, namely glucose...
متن کاملUseful characteristics and commercial applications of fructo-oligosaccharides.
Introduction Fructo-oligosaccharides consist of several fructosyl residues linked to a terminal glucose moiety. They occur naturally in many plant species, such as onion, edible burdock, asparagus, wheat and so on. These fructo-oligosaccharides are commercially produced from sucrose by the transfructosylation reaction of an enzyme or from inulin by enzymatic hydrolysis. This paper reviews the b...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Carbohydrate polymers
دوره 136 شماره
صفحات -
تاریخ انتشار 2016